In order to complete our portfolio, IAB now offers Sanger sequencing – a reliable method long-term and widely used for analysis of primary structure of short DNA sequences, such as PCR amplicons. Fluorescent-labelled dideoxynucleotides (ddNTP) incorporated during DNA replication are read precisely one by one in sequencer using the principle of capillary electrophoresis. This method is cost-effective, readily available, and often preferred due to its well-established protocols.
